Skip to main content
. 2010 Dec 7;30(2):302–316. doi: 10.1038/emboj.2010.312

Table 2. Plasmids used in this study.

Yeast plasmid Relevant feature Source
pBAS2004 TIF4632 URA3 CEN4 Tarun and Sachs (1996)
pBAS2078 TIF4631 TRP1 CEN4 Tarun and Sachs (1997)
pBAS3157 TIF4631-HA TRP1 CEN4 Tarun and Sachs (1997)
pEP88 TIF4631-HA-Bam TRP1 CEN4 This study
pEP206 tif4631-HA-Bam-Δ1–300 TRP1 CEN4 This study
pBAS3158 tif4631-HA-459 TRP1 CEN4 Tarun and Sachs (1997)
pEP112 tif4631-HA-Bam-459197–224 TRP1 CEN4 This study
pEP87 tif4631-HA-Bam-45994–187 TRP1 CEN4 This study
pEP86 tif4631-HA-Bam-45994–300 TRP1 CEN4 This study
pEP82 tif4631-HA-459188–300 TRP1 CEN4 This study
pEP46 tif4631-HA-213,–459 TRP1 CEN4 This study
pEP130 tif4631-HA-Bam-45994–111 TRP1 CEN4 This study
pEP131 tif4631-HA-Bam-459128–150 TRP1 CEN4 This study
pBAS3120 tif4631–213 TRP1 CEN4 Tarun and Sachs (1997)
pEP187 tif4631-HA-Bam-213,–45994–111 TRP1 CEN4 This study
pEP188 tif4631-HA-Bam-213,–459128–150 TRP1 CEN4 This study
pEP93 tif4631-HA-Bam-4591–82 TRP1 CEN4 This study
pEP205 tif4631-HA-Bam-4591–300 TRP1 CEN4 This study
pEP102 tif4631-HA-Bam-4591–82188–300 TRP1 CEN4 This study
pEP103 tif4631-HA-Bam-459492–539 TRP1 CEN4 This study
pEP104 tif4631-HA-459883–952 TRP1 CEN4 This study
pEP177 tif4631-HA-Bam-Δ1–82 TRP1 CEN4 This study
pEP58 tif4631-Δ1–187 TRP1 CEN4 Tarun et al (1997)
pEP113 tif4631-HA-Bam-Δ492–539 TRP1 CEN4 This study
pEP199 tif4631-HA-Δ883–952 TRP1 CEN4 This study
pEP148 tif4631-HA-Bam-4591–187 TRP1 CEN4 This study
pEP207 tif4631-HA-Bam-213,–4591–82 TRP1 CEN4 This study
pEP201 tif4631-HA-Bam-213,–459492–539 TRP1 CEN4 This study
pEP202 tif4631-HA-213,–459883–952 TRP1 CEN4 This study
pEP115 tif4631-HA-Bam-4591–82197–224 TRP1 CEN4 This study
pEP59a tif4631–4591–187 TRP1 CEN4 Tarun et al (1997)
pEP60b tif4631-Δ1–300 TRP1 CEN4 Tarun et al (1997)
pEPB1c PT7lac-GST-TIF46312–300-His6 PT7lac-CDC33 This study
pEPB6c PT7lac-GST-tif46312–3001–82 (RNA1)-His6 PT7lac-CDC33 This study
pEPB7c PT7lac-GST-tif46312–30094–111 (ΔB1)-His6 PT7lac-CDC33 This study
pEPB8c PT7lac-GST-tif46312–300128–150 (ΔB2)-His6 PT7lac-CDC33 This study
pEPB9c PT7lac-GST-tif46312–300197–224 (ΔB3)-His6 PT7lac-CDC33 This study
pEPB10c PT7lac-GST-tif46312–30094–187 (Δ[B1,B2ex])-His6 PT7lac-CDC33 This study
pEPB11c PT7lac-GST-tif46312–300–213-His6 PT7lac-CDC33 This study
pEPB12c PT7lac-GST-tif46312–3001–187-His6 PT7lac-CDC33 This study
aThis plasmid was rescued from yeast strain YAS2104 (Tarun et al, 1997).
bThis plasmid was rescued from yeast strain YAS2071 (Tarun et al, 1997).
cThe different TIF4631 fragments were inserted at the BamHI and HindIII sites of pEPB1. Bacterial strain BL21-CodonPlus (DE3)-RIL was transformed with the indicated plasmids.