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. 2010 Dec 7;30(2):302–316. doi: 10.1038/emboj.2010.312

Table 3. S. cerevisiae strains used in this study.

Strain Genotype Source
YAS2282 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg Tarun and Sachs (1997)
  tif4632∷ura3 pBAS2004 [pTIF4632 URA3 CEN4]  
YAS2069 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷LEU2 Tarun and Sachs (1997)
  tif4632∷ura3 pBAS2078 [pTIF4631 TRP1 CEN4]  
EPY88 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP88 [TIF4631-HA-Bam TRP1 CEN4]  
EPY206 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP206 [tif4631-HA-Bam-Δ1–300 TRP1 CEN4]  
EPY52a MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg Tarun and Sachs (1997)
  tif4632∷ura3 pBAS3158 [tif4631-HA-459 TRP1 CEN4]  
EPY112 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP112 [tif4631-HA-Bam-459,Δ197–224 TRP1 CEN4]  
EPY87 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP87 [tif4631-HA-Bam-459,Δ94–187 TRP1 CEN4]  
EPY86 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP86 [tif4631-HA-Bam-459,Δ94–300 TRP1 CEN4]  
EPY82 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP82 [tif4631-HA-459,Δ188–300 TRP1 CEN4]  
EPY46 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP46 [tif4631-HA-213,–459 TRP1 CEN4]  
EPY130 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP130 [tif4631-HA-Bam-459,Δ94–111 TRP1 CEN4]  
EPY131 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP131 [tif4631-HA-Bam-459,Δ128–150 TRP1 CEN4]  
YAS2075 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷LEU2 Tarun and Sachs (1997)
  tif4632∷ura3 pBAS3120 [tif4631–213 TRP1 CEN4]  
EPY187 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP187 [tif4631-HA-Bam-213,–459,Δ94–111 TRP1 CEN4]  
EPY188 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP188 [tif4631-HA-Bam-213,–459,Δ128–150 TRP1 CEN4]  
EPY93 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP93 [tif4631-HA-Bam-459,Δ1–82 TRP1 CEN4]  
EPY132 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632::ura3 pEP88 [TIF4631-HA-Bam TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
EPY133 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pBAS3158 [tif4631-HA-459 TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
     
EPY205 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP205 [tif4631-HA-Bam-459,Δ1–300 TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
EPY157 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632∷ura3 This study
  pEP93 [tif4631-HA-Bam-459,Δ1–82 TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
EPY158 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632∷ura3 This study
  pEP82 [tif4631-HA-459,Δ188–300 TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
EPY102 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632∷ura3 This study
  pEP102 [tif4631-HA-Bam-459,Δ1–82,Δ188–300 TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
EPY103 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632∷ura3 This study
  pEP103 [tif4631-HA-Bam-459,Δ492–539 TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
EPY104 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632∷ura3 This study
  pEP104 [tif4631-HA-459,Δ883–952 TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
EPY161b MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg Tarun and Sachs (1997)
  tif4632∷ura3 pBAS2004 [pTIF4632 URA3 CEN4]  
  pBAS2078 [pTIF4631 TRP1 CEN4]  
     
EPY203 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632∷ura3 This study
  pEP46 [tif4631-HA-213,–459 TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
EPY200 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632∷ura3 This study
  pEP207 [tif4631-HA-Bam-213,–459,Δ1–82 TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
EPY201 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632∷ura3 This study
  pEP201 [tif4631-HA-Bam-213,–459,Δ492–539 TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
EPY202 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632::ura3 This study
  pEP202 [tif4631-HA-213,–459,Δ883–952 TRP1 CEN4]  
  pBAS2004 [pTIF4632 URA3 CEN4]  
EPY177 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP177 [tif4631-HA-Bam-Δ1–82 TRP1 CEN4]  
EPY58c MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg  
  tif4632::ura3 pEP58 [tif4631-Δ1–187 TRP1 CEN4]  
EPY113 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632∷ura3 This study
  pEP113 [tif4631-HA-Bam-Δ492–539 TRP1 CEN4]  
EPY199 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631::leu2hisg tif4632∷ura3 This study
  pEP199 [tif4631-HA-Δ883–952 TRP1 CEN4]  
EPY148 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg This study
  tif4632∷ura3 pEP148 [tif4631-HA-Bam-459,Δ1–187 TRP1 CEN4]  
EPY207 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632∷ura3 This study
  pEP207 [tif4631-HA-Bam-213,–459,Δ1–82 TRP1 CEN4]  
EPY115 MATa ade2 his3 leu2 trp1 ura3 pep4∷HIS3 tif4631∷leu2hisg tif4632∷ura3 This study
  pEP115 [tif4631-HA-Bam-459,Δ1–82,Δ197–224 TRP1 CEN4]  
aThe plasmid pBAS3158 was transformed into yeast strain YAS2282.Transformants were plated on 5-fluoroorotic acid plates lacking tryptophan (FOA-W) to eliminate the plasmid expressing wild-type TIF4632.
bThe plasmid pBAS2078 was transformed into yeast strain YAS2282.
cThe plasmid pEP58 was rescued from yeast strain YAS2070 (Tarun et al, 1997) and transformed into yeast strain YAS2282.Transformants were plated on FOA-W plates to eliminate the plasmid expressing wild-type TIF4632.