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. 2010 Dec 14;30(2):379–394. doi: 10.1038/emboj.2010.326

Figure 1.

Figure 1

Impaired A-SMase activation and apoptosis after TNF stimulation in caspase-8-deficient Jurkat cells. (A) Time course of A-SMase activity determined in Jurkat cell lysates after TNF treatment. Wild-type Jurkat cells are compared with caspase-8-deficient (caspase-8 knockout) Jurkat cells as well as to caspase-8-deficient Jurkat re-transfected with caspase-8 expression plasmid (caspase-8 reconstituted) and caspase-8-deficient Jurkat mock-transfected with an empty plasmid (caspase-8 mock transf.). (B) Time course of ceramide production determined in Jurkat cell lysates after TNF treatment. Wild-type Jurkat cells are compared with caspase-8-deficient Jurkat cells. Data of two experiments are shown, each performed in triplicates (±s.e.m.). (C) Induction of apoptosis in Jurkat wild-type and caspase-8-deficient cells after treatment with 0.5 μg/ml CHX and 100 ng/ml TNF for 4 h, analysed by annexin-V/propidium iodide staining.