Table 3.
Influence of Point Mutations in ApoE3 and ApoE4 on Binding to Lipoprotein Particles
| ApoE variant | Relative binding a |
|
|---|---|---|
| VLDL | HDL3 | |
| apoE3 | ||
| WT | 1.0 | 1.0 |
| K146E | 2.1 ± 0.5 | 1.2 ± 0.3 |
| K146Q | 2.6 ± 0.5 | 1.0 ± 0.2 |
| P267A | 0.5 ± 0.1 | 0.5 ± 0.1 |
| G278P | 0.7 ± 0.2 | 0.6 ± 0.2 |
| apoE4 | ||
| WT | 1.0 | 1.0 |
| E255A | 0.4 ± 0.1 | 0.5 ± 0.1 |
| G278P | 0.8 ± 0.1 | 0.6 ± 0.1 |
Fractional binding of the apoE variants was determined using a VLDL/HDL3 distribution assay under defined conditions (see Experimental Procedures) and is expressed relative to the value for the respective WT protein. The values are mean ± SEM.