Table 1. Summary of genetic interactions with myo2 tail mutants.
| Allele | Restrictive temperature1 |
Over- expression of PIK12 |
Combined with pik1-139 |
Combined with sac1Δ |
Expression of myo2ΔCTD-PHWT3 |
Expression of myo2ΔCTD-PHEE3 |
Over- expression of SEC44 |
Over- expression of YPT314 |
|---|---|---|---|---|---|---|---|---|
| MYO2+ | >37°C | NE | 36°C | NE | NE | NE | NE | NE |
| myo2-12 | 33°C | 35°C | 30°C | 37°C | >37°C | 30°C | 36°C | 35°C |
| myo2-13 | 35°C | NE | NE | >37°C | 37°C | 33°C | NE | NE |
| myo2-14 | 35°C | NE | NE | NE | NE | 33°C | NE | NE |
| myo2-16 | 32°C | NE | 30°C | 36°C | >37°C | 30°C | 35°C | NE |
| myo2-17 | 37°C | NE | NE | NE | NE | NE | NE | NE |
| myo2-18 | 36°C | NE | NE | 37°C | 37°C | NE | NE | NE |
| myo2-20 | 37°C | NE | NE | NE | NE | NE | NE | NE |
The highest temperatures showing growth are reported for strains with either wild-type MYO2, or myo2 alleles with mutations in the C-terminal domain (Schott et al., 1999). NE, no effect
1
Restrictive temperature on synthetic complete medium.
2
PIK1 was expressed behind the GAL1 promoter.
3
The fusion constructs were expressed from the MYO2 promoter.
4
SEC4 and YPT31 were expressed on a low copy plasmid from the ADH1 promoter.