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RT–PCR validations of splicing changes for alternative splicing events with high co-regulation values; (A) F42G9.6, (B) pqn-52, (C) C06A6.4, (D) lec-3 and (E) hrpf-1. An Agilent Bioanalyzer 2100 was used to quantitate the experimental results, error bars are shown representing results from at least two different RT–PCR reactions, and an electropherogram for each experiment is shown.
