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Substrate preference of wild-type EndA: Nucleolytic activity of wild-type EndA was tested on (A) ΦX174 DNA (single-stranded, circular), (B) RF I DNA (double-stranded, circular) and (C) RF II DNA (double-stranded, nicked). Wild-type EndA shows no substrate preference (ss—single stranded, sc—super coiled, oc—open circle, li—linear).
