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Overexpression of PCAF stimulates XBP-1S-mediated transcription. HEK293 cells were transiently co-transfected with a luciferase reporter [(A) HTLV-Luc and (B) BiP-Luc] and indicated expression plasmids (i.e. XBP-1S, PCAF and p300). The amounts of PCAF and p300 plasmids were titrated at 3-fold increment. The total amounts of plasmids transfected were kept constant by adjusting the mock vector. *P < 0.05 versus control (i.e. cells transfected with a XBP-1S expression plasmid).
