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. 2010 Sep 4;39(2):429–439. doi: 10.1093/nar/gkq785

Figure 4.

Figure 4.

Knockdown of PCAF inhibits XBP-1S-mediated transcription. (A) Cell lysates of the PCAF siRNA-transfected HEK293 cells were analyzed by western blotting to determine the effectiveness of the siRNAs. Cells transfected with a non-specific siRNA were used as a negative control (i.e. Con.). For the luciferase-based assays, HEK293 cells were transiently co-transfected with a luciferase reporter [(B) HTLV-Luc and (C) BiP-Luc], a XBP-1S expression plasmid, and an indicated siRNA. The siRNAs used for the experiments included non-specific (i.e. Con.), luciferase (i.e. GL3), two PCAF (i.e. PCAF-6 and PCAF-7) and two p300 (i.e. p300-7 and p300-10) siRNAs. *P<0.05 versus control (i.e. cells co-transfected with a control non-specific siRNA and a XBP-1S expression plasmid).