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. 2010 Sep 4;39(2):429–439. doi: 10.1093/nar/gkq785

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Knockdown of PCAF inhibits the transcription of endogenous XBP-1S target genes. (A) MCF7 cells were co-transfected with the indicated plasmid (i.e. empty or XBP-1S expression vectors) and shRNA [i.e. non-specific (Con.) or PCAF]. Expression of PCAF and XBP-1S was analyzed by western blotting. (B) The mRNAs of the XBP-1S target genes, including BiP, CHOP and EDEM were quantified by QRT–PCR. Cells transfected with an empty vector and a non-specific shRNA served as a negative control. Knockdown of PCAF using a specific PCAF shRNA exhibited significant decreases in the expression of BiP CHOP, and EDEM, respectively (*P < 0.05 versus cells co-transfected with a control non-specific shRNA and a XBP-1S expression plasmid).