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XBP-1S recruits PCAF to the target genes of XBP-1S in vivo. MCF7 cells were co-transfected with a PCAF expression vector and an indicated plasmid [i.e. empty (pcDNA6), XBP-1U, or XBP-1S]. ChIP was carried out followed by quantitative PCR to quantify the binding of XBP-1S and PCAF to the promoters of BiP (A) and CHOP (B). Cells transfected with an empty vector and a PCAF plasmid was used as a negative control. *P < 0.05 versus negative controls.
