Figure 8.

Requirement of PCAF for the mediation of XBP-1S target genes under UPR. MCF7 cells were co-transfected with a non-specific (i.e. control) or PCAF shRNA, and incubated with 10 µg/ml Tm (A) or 300 nM Tg (B). Both Tm and Tg were dissolved in DMSO and the final concentration of DMSO in the culture was kept at 0.1%. Expression of endogenous BiP and CHOP genes was determined by QRT–PCR. Cells transfected with a control shRNA with 0.1% DMSO were served as a negative control. For quantitative ChIP assays, HEK293 cells were treated with 10 µg/ml Tm (C) or 300 nM Tg (D) and the bindings of XBP-1S and PCAF to the endogenous BiP and CHOP genes were analyzed by quantitative PCR. Cells incubated with 0.1% DMSO were used as a negative control. Fold changes were determined by comparing to the negative controls. *P < 0.05 versus negative controls.