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Interaction between XBP-1S and PCAF under UPR. 293T cells were transiently transfected with a XBP-1S expression vector and incubated with 10 µg/ml Tm or 0.1% DMSO (i.e. the negative control) for 16 h. IP was performed using the cell lysates prepared from the transfected cells and the antibody against XBP-1. Normal IgG (IgG) was used as a negative control. The immunoprecipitated complexes and the protein inputs were analyzed by western blotting.
