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. 2010 Sep 17;39(2):464–474. doi: 10.1093/nar/gkq809

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Replacement of TFIIB linker sequences. Stimulatory activities of a variety of artificial [glycine-serine (‘G-S’) repeats], archaeal (Halobacterium salinarum TFB-A; Methanopyrus kandleri) and eukaryotic (Saccharomyces cerevisiae; Drosophila melanogaster; Homo sapiens) TFIIB-linkers after replacement of the orthologous sequence of mjTFIIB. The primary sequences of the hybrids are shown below with the replaced TFIIB-linker sequence highlighted in bold. The activity of each mutant was calculated relative to the mjTFIIB wild-type stimulation rate valued as 100%. Each mutant was assessed in triplicate (error bars represent standard deviations).