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. 2010 Sep 10;39(2):440–453. doi: 10.1093/nar/gkq796

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Regulation of miRNA-107 and PANK1 protein after knockdown of p53. (A) HCT116 p53^+/+ cells were transfected with a p53-targeting siRNA (p53) or control siRNA (control). After 24 h, cells were treated with doxorubicin for different time periods. miRNA-107 was measured and normalized to the snRNA U6 control. RNA expression was calculated as the log₂ transformed ratio relative to the 0 h time point. (B) Western-blot analyses of PANK1 after 48 h of doxorubicin treatment. As a control, p53, p21^CIP1/WAF1 and GAPDH were detected on the same blot.