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. 2010 Sep 10;39(2):440–453. doi: 10.1093/nar/gkq796

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Regulation of CDK6 and p130 gene expression by miRNA-107. (A) D53wt cells were transfected with increasing amounts of pre-miRNA-107, with a control-pre-miRNA (control) or without RNA (mock). For knockdown experiments D53wt cells were transfected with AntagomiR-107 and a related control. CDK6 protein was detected by western analyses from whole cell lysates. GAPDH expression served as a loading control. (B) CDK6 mRNA was measured by real-time RT–PCR in transfected D53wt cells. Expression values from three independent experiments were normalized to GAPDH expression. (C) Western analysis of p130 protein expression in transfected D53wt cells and GAPDH control. (D) The p130 mRNA was measured in total RNA samples from transfected D53wt cells. (E) and (F) As a control, miRNA-107 levels in controls and pre-miRNA-107- or AntagomiR-107-transfected cells were assayed. Averages and standard deviations from three independent experiments are shown.