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. 2010 Sep 17;39(2):536–544. doi: 10.1093/nar/gkq791

Figure 3.

Figure 3.

Cleavage activity of Tma endo V on 5′-labeled single-stranded and short I-containing substrates. (A) Exonuclease activity of Tma endo V mutants on 5′-labeled single stranded I-containing substrate. Cleavage reactions were performed as described in ‘Materials and Methods’ section with 100 nM enzyme and 10 nM substrate. (B) Exonuclease activity of Tma endo V mutants on 5′-labeled short I-containing substrate. Cleavage reactions were performed as described in ‘Materials and Methods’ section with 100 nM enzyme and 10 nM substrate.