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. 2010 Sep 17;39(2):536–544. doi: 10.1093/nar/gkq791

Figure 8.

Figure 8.

Time course analysis of cleavage activity by wt Tma endo V on 3′-labeled full-length T/A substrate. Cleavage reactions were performed as described in ‘Materials and Methods’ section with 100 nM enzyme and 10 nM substrate. Reactions were stopped on ice at the indicated time points, followed by addition of an equal volume of GeneScan stop buffer. (A) Time course analysis of cleavage activity by wt Tma endo V on 3′-labeled full-length T/A substrate. (B) Plot of time course analysis of cleavage activity by wt Tma endo V on 3′-labeled full-length T/A substrate. The experimental errors were estimated to be <10%. The cleavage data were fitted into a first-order equation using DeltaGraph version 5 (SPSS Inc. and Red Rock Software Inc.).