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. 2010 Sep 18;39(2):502–512. doi: 10.1093/nar/gkq820

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Cyclical induction of HDAC4 and HDAC6 transcription by 1α,25(OH)₂D₃. Real-time quantitative PCR was performed in order to measure the time-dependent mRNA expression of the genes HDAC4 (A and B) and HDAC6 (C and D) in MCF-10A cells after treatment with 10 nM 1α,25(OH)₂D₃ (A and C) or 10 nM Gemini (B and D). The data were normalized to the expression of the housekeeping gene RPLP0 and fold inductions were calculated in reference to vehicle control. Data points indicate the means of at least three independent cell treatments and the bars represent standard deviations. A two-tailed Student’s t-test was performed to determine the significance of the stimulation in reference to vehicle-treated control and for the comparison of the peaks to the minima (*P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant).