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. 2010 Sep 15;39(2):675–686. doi: 10.1093/nar/gkq776

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Sno-miRNA analysis in human HeLa and Jurkat cells. Five individual snoRNAs were probed for expression and processing via northern blots with total RNA extracts from HeLa and Jurkat cells as shown in (B). Processed sno-miRNAs can be identified in both cell types. In Hela extracts sno-miRNAs from U3, U78 and HBI-43 can be seen, in Jurkat cell extracts the sno-miRNAs U74 and U78 were identified. The total sizes of full length snoRNAs are indicated. Reporter gene assays with complementary target sites for the sno-miRNAs indicate a correlation of snoRNA processing to gene silencing activity as displayed in (A). A randomized non-cognate target sequence served as negative control, the ACA45 sno-miRNA as a positive control. The empty psiCHECK-2 vector was used for normalization. Data represent the average outcome of four (HeLa, Jurkat) or three (RPMI8866, MCF7) individual experiments.