Table 1.
snoRNA derived sRNAs (sno-miRs), positions on original snoRNA sequences and miRNA-like activity
| snoRNA | HGNC symbol (host gene) | Type | Sno-miR pos. (major) | Sno-miR *pos. (minor) | miRNA activity (H/J/R) | References |
|---|---|---|---|---|---|---|
| ACA45 | SCARNA15 (Hs.513091) | H/ACA | 64–85 | 102–127 | +/+/O | (16) |
| snR39b | SNORD2 (EIF4A2) | C/D | 2–29 | 36–63 | +/+/− | |
| U3 | X14945 | C/D | 193–214 | (93–127) | +/O/+ | |
| U3-3 | SNORD3 | C/D | 45–66 | −/O/n | ||
| U3-4 | SNORD3 | C/D | 75–96 | O/+/+ | ||
| U78 | SNORD78 (GAS5) | C/D | 32–61 | 1–24 | +/+/O | |
| HBII-336 | SNORD93 (EST cluster) | C/D | 40–62 | 2–23 | −/+/+ | This study |
| HBI-43 | SNORD17 (SNX5) | C/D | 207–233 | 2–29 | O/–/n | |
| HBII-142 | SNORD66 (EIF4G1) | C/D | 47–70 | 1–23 | O/+/n | |
| U48 | SNORD48 (C6orf48) | C/D | 1–25 | 40–61 | −/O/n | |
| U51 | SNORD51 (EEF1B2) | C/D | 41–65 | 2–39 | −/O/n | |
| U21 | SNORD21 (RPL5) | C/D | 2–27 | 52–86 | −/−/n | |
| U27 | SNORD27 (U22 Host Gene, UHG) | C/D | 43–67 | 2–24 | −/+/− | |
| U44 | SNORD44 (AL110141) | C/D | 2–26 | 28–57 | −/O/n | |
| HBII-429 | SNORD100 (RPS12) | C/D | 4–36 | 37–70 | +/O/n | |
| U59b | SNORD59b (ATP5B) | C/D | 44–67 | 6–25 | −/O/n | |
| U83a | SNORD83a (RPL3) | C/D | 4–26 | n.d. | −/+/+ | |
| U15a | SNORD15a (RPS3) | C/D | 2–27 | 114–137 | +/+/n | |
| U74 | SNORD74 (GAS5) | C/D | 40–68 | 3–30 | −/+/n | |
| U45AC | SNORD45aSNORD45c (RABGGTB) | C/D | 57–73 | 1–29 | O/−/n | |
| miRbase listed snoRNAs | Identified | |||||
| ACA34 hsa-miR-1291 | SNORA34 (FLJ20436) | H/ACA | 2–25 | (39–63) | +/+/+ | (35) |
| HBI-61 hsa-miR-1248 | SNORA81 (EIF4A2) | H/ACA | 1–27 | n.d. | +/+/+ | |
| ACA36b hsa-miR-664 | SNORA36b | H/ACA | 106–128 | 68–91 | +/O/− | |
| HBII-99b hsa-miR-1259 | SNORD12b (C20orf199) | C/D | 16–36 | n.d. | n.d. | This study |
| SNORD126 hsa-miR-1201 | SNORD126 (CCNB1IP1) | C/D | 52–75 | n.d. | n.d. | |
Host gene, pre-mRNA source of intron-encoded snoRNA; sno-miR, small RNA originating from snoRNAs; major, dominant sRNA, found in high copy numbers by deep sequencing; minor, sRNA found in significantly lower copy numbers; var., various minor sRNAs; n.d., not detected; miRNA activity: HeLa, Jurkat, RPMI8866 +, <60% remaining Renilla luciferase activity; O, 60–80%; −, no reduction.