Figure 4.

Binding and cleavage data. (A) Fluorescence anisotropy results for OkrAI and BamHI. The fraction of the bound DNA is plotted against the concentration of the protein. The data points for BamHI are shown as black half squares and for OkrAI as red half circles, and the fitted curves as solid lines. (B) Cleavage activity data for OkrAI (top) and BamHI (bottom) at 25°C and 37°C using λ DNA as the substrate. The fidelity index (FI) is defined as the ratio of the maximum enzyme amount showing no star activity to the minimum amount needed to achieve 1 U of activity.