Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Outline of the tandem-affinity-immunopurification strategy. An artificial adaptor protein combines an IGB with a calmodulin-binding peptide (C) and a strep tag (S). The adaptor is used to immobilize antibodies on calmodulin beads, which are then incubated with extracts containing the antigen. Immunocomplexes are recovered by native elution with EGTA and are re-purified on Streptactin beads.
