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. 2010 Nov 4;39(2):e7. doi: 10.1093/nar/gkq966

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Leak expression of Cre in E. coli. Representations are the same as in Figure 1 unless otherwise stated. (a) Structure of cosmid generating double-unit AdV and expressing dnCreRY. Ψ, adenovirus packaging sequences; P trc, trc promoter; t, terminator. (b) Generation of Cre-processed molecules. A, AhdI site. (c) Detection of Cre-processed molecules. M, 1-kb ladder marker; mn, mini-preparation; md; midi-preparation. The bands of 2.7 and 2.5 kb represent the generated circular DNA and Cre-processed DNA, respectively.