Figure 1.

AbrB and Abh interaction in vivo. Western blot analysis of exponentially growing B. subtilis cells, either untreated or treated with 2 mM DSP for 5, 15 or 25 minutes. Lysates of cells expressing AbrB-2HC in the abh⁺ or Δabh background were probed using anti-AbrB antibody (A) or anti-Abh antibody (B). Cells expressing Abh-2HC in the abrB⁺ or ΔabrB background were probed using anti-Abh antibody (C). Electrophoretic mobilities of AbrB/Abh monomers, dimers, and heteromers, as well as those of proteins in a pre-stained broad-range marker mix (Bio-Rad), are indicated.