Figure 2.

Confirmation of Aβ42 conjugation to AuNP and induction of AuNP–Aβ42 precipitates by Aβ42 aggregation. A) Verification of biotin–Aβ42 conjugation to streptavidin-AuNP by dot blot analysis. B) After 48 hours incubation, visible AuNP–Aβ42 precipitates formed in the bottom of the tubes and the supernatant became clear; no precipitate was observed in the AuNP control. C) Optical density of supernatant as a quantitative indicator of AuNP–Aβ42 precipitation (Paired t-test, ***P < 0.0001). D) ThT binding assay to measure and quantify Aβ42 aggregation, reflected by significantly increased ThT binding in AuNP–Aβ42 (**P < 0.005).

Abbreviations: Aβ42, amyloid β42; AuNP, gold nanoparticle; ThT, thioflavin T.