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. Author manuscript; available in PMC: 2011 Oct 1.

Published in final edited form as: Circ Res. 2010 Aug 12;107(7):877–887. doi: 10.1161/CIRCRESAHA.110.222968

Lysine 609 mediates aspirin-stimulated binding of eNOS to calmodulin. A through C, Aspirin rapidly stimulates binding of eNOS to calmodulin. Coimmunoprecipitation of (A) calmodulin (CaM) in eNOS immunoprecipitates and (B) eNOS in CaM immunoprecipitates from HEK 293 cells expressing ectopic eNOS and FLAG-CaM treated with a low concentration of ASA for the indicated times. C, Coimmunoprecipitation of endogenous CaM in eNOS immunoprecipitates and endogenous eNOS in CaM immunoprecipitates from HUVEC, with and without treatment with ASA. D, Lysine 609 in bovine eNOS mediates its aspirin-stimulated binding to CaM. Coimmunoprecipitation of FLAG-CaM with eNOS (WT) and eNOS (K609R) ectopically expressed in HEK 293 cells treated with ASA. Representative immunoblots are shown with accompanying analysis by densitometry of immunoprecipitated CaM/eNOS and eNOS/CaM relative to no aspirin from independent blots. *P<0.05, **P<0.01, and #P=NS (n=3 to 4).

Lysine 609 mediates aspirin-stimulated binding of eNOS to calmodulin. A through C, Aspirin rapidly stimulates binding of eNOS to calmodulin. Coimmunoprecipitation of (A) calmodulin (CaM) in eNOS immunoprecipitates and (B) eNOS in CaM immunoprecipitates from HEK 293 cells expressing ectopic eNOS and FLAG-CaM treated with a low concentration of ASA for the indicated times. C, Coimmunoprecipitation of endogenous CaM in eNOS immunoprecipitates and endogenous eNOS in CaM immunoprecipitates from HUVEC, with and without treatment with ASA. D, Lysine 609 in bovine eNOS mediates its aspirin-stimulated binding to CaM. Coimmunoprecipitation of FLAG-CaM with eNOS (WT) and eNOS (K609R) ectopically expressed in HEK 293 cells treated with ASA. Representative immunoblots are shown with accompanying analysis by densitometry of immunoprecipitated CaM/eNOS and eNOS/CaM relative to no aspirin from independent blots. *P<0.05, **P<0.01, and #P=NS (n=3 to 4).