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. 2010 Sep 1;278(1705):546–553. doi: 10.1098/rspb.2010.1301

Figure 1.

Figure 1.

Myxozoan polar capsule, PCR profiles, minicollagen phylogeny and minicollagen gene organization. (a) Transmission electron micrograph of polar capsule of an undescribed but close relative of T. bryosalmonae showing the capsule wall with characteristic electron-dense and lucent regions (arrows), the coiled inverted filament (if), the capsule matrix (cm), the base of the inverted filament (b) and thin valve cells overlying the capsulogenic cell (cc) except where the polar filament discharges. Scale bar, 0.33 µm. (b) PCR profile showing the presence of Tb-Ncol-1 in control (−P) and T. bryosalmonae-infected (+P) bryozoan (top) and rainbow trout (bottom) cDNA and genomic DNA. Lane 1, negative control (water as template). M, size marker. (c) Unrooted BI tree based on the alignment of 17 known group 1 minicollagen sequences using full-length amino acid sequences. Posterior probabilities above nodes. Bootstrap values for NJ and ML analysis below nodes with black circles. Amino acid sequence identity/similarity to Tb-Ncol-1 is on the right. Sequences partition into three groups representing known hydrozoan and cubozoan sequences with a single Gly-X-Y domain (clade A), hydrozoan sequences with a double Gly-X-Y domain (clade B) and all known anthozoan group 1 minicollagens (clade C). (d) Gene organization of known group 1 minicollagens and Tb-Ncol-1 based on sequences from the initiating ATG codon in exon I. Coding regions are represented as solid boxes. Numbers refer to corresponding amino acid number. Light grey, white and dark grey boxes represent the signal peptide, propeptide and the mature minicollagen peptide, respectively. Solid bar is the single intron within the ORF of each gene (number of basepairs below). Note conservation of the intron phase as indicated by the interrupted codon fraction. Nematostella genomic sequences obtained as in figure 2. Hydra magnipapillata genomic sequences obtained from NCBI genomes database with the genome scaffold numbers and first basepair positions as follows: Hm-Ncol-1, NW_002189376 (position 297); Hm-Ncol-2, NW_002163809 (position 52 189); Hm-Ncol-12, NW_002164275 (position 113 860); Hm-Ncol-5, NW_002161445 (position 9789); Hm-Ncol-6, NW_002160847 (position 89071).