Figure 2.

Multiple alignment of the predicted Tb-Ncol-1 translation with known group 1 minicollagens. Gaps introduced to increase identity are shown by dashes. Highlighted features include the cysteine arrangement in the N- and C-terminal CRDs. Brackets indicate spatial differences between cysteines in the N-terminal and C-terminal CRDs between Tb-Ncol-1 and the canonical CRDs in other group 1 minicollagens. Predicted signal peptides, the conserved KR endopeptidase cleavage site, non-helical alanines and residues directing the three-dimensional structure, and disulphide arrangements in both CRDs are highlighted. Glycine residues in the two collagen triple helix domains are in bold. Abbreviations: Hm, Hydra magnipapillata; Ch, Clytia hemisperica; He, Hydractinia eichinata; Mk, Malo kingi; Ho, Hydra oligactis; Pd, Podocoryne carnea; Ms, Metridium senile; Nv, Nematostella vectensis; Tb, Tetracapsuloides bryosalmonae. Sequences obtained from NCBI protein and EST databases under the following accession nos: Hm-Ncol-1, CAA43379; Hm-Ncol-2, CAA43380; Hm-Ncol-5, CF656203; Hm-Ncol-6, CF658139; Ch-Ncol-1, ABY71252; He-Ncol-1, DT621782; Mk-Ncol-1, ACN93684; Ms-Ncol-1, FC836938; Ms-Ncol-4, FC827212; Ho-Ncol-1, C0K091; Pd-Ncol-1, DY450665; Hm-Ncol-11, BP512837; Hm-Ncol-12, TC3264; Hm-Ncol-7, TC066. The genome scaffold and first base pair positions of the Nematostella minicollagens are: Nv-Ncol-3, scaffold 23 position 1 380 889; Nv-Ncol-4, scaffold 5 position 998 944; Nv-Ncol-5, scaffold 5 position 993 833.