Table 1.
Validation of microarray leaf and root data by real-time RT-PCR of seven genes identified as significantly regulated from leaf or root hybridizations
| Description | Rice match | Putative function | Array |
RT-PCR |
||
|---|---|---|---|---|---|---|
| Cashel-P | IRL-OP-02538-P | Cashel-P | IRL-OP-02538-P | |||
| Leaf | ||||||
| U35-44k-v1-12109 | LOC-Os02g33710·1 | Histidine decarboxylase | 5·9 (0·001) | 5·8 (0·000) | 8·2 (0·000) | 7·4 (0·000) |
| U35-44k-v1-37470 | LOC-Os12g37600·1 | Glycerol-3-phosphate acyltransferase 1 | 0·9 (0·951) | 5·8 (0·005) | 0·9 (0·904) | 2·7 (0·029) |
| U35-44k-v1-8347 | LOC-Os03g30130·2 | Phospholipase C | 2·8 (0·007) | 4·3 (0·006) | 1·4 (0·374) | 1·6 (0·178) |
| U35-44k-v1-7335 | LOC-Os01g54620·1 | CESA4 – cellulose synthase | 1·4 (0·702) | 7·0 (0·038) | 1·2 (0·626) | 4·0 (0·013) |
| Root | ||||||
| U35-44k-v1-22715 | LOC-Os01g67126·1 | 60S ribosomal protein L5-2 | 3·3 (0·585) | 3·6 (0·032) | 0·6 (0·714) | 8·2 (0·000) |
| U35-44k-v1-49610 | LOC-Os08g43870·1 | Hypothetical protein | 1·0 (0·939) | 2·6 (0·024) | 0·9 (0·543) | 2·8 (0·003) |
| U35-44k-v1-43490 | LOC-Os08g39300·1 | Serine-glyoxylate aminotransferase | 1·2 (0·718) | 0·3 (0·021) | 0·7 (0·487) | 0·3 (0·000) |
The fold change for both array and RT-PCR data are shown with associated significance values in parentheses; significant differences in bold. Array data were analysed in GeneSpring and RT-PCR data in REST.