Fig. 5. Gαi amino acid sequences for the trout saccular hair cell preparation were deduced from nucleotide sequencing of cloned 1,016 bp and 771 bp PCR products.
The 1,016 bp cloned product (line 1), representing 95% of the coding region for a Gαi protein, bore highest amino acid identity to medaka Gαi1 (line 2, NP_001116402) and rat Gαi1 (line 3, NP_037277). A second cloned product 771 bp in length (line 4) was highest in amino acid identity to Salmo salar Gαi3 sequence (line 5) (NP_001135256) and rat Gαi3 (line 6, NP_037238).