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. Author manuscript; available in PMC: 2011 Jan 24.
Published in final edited form as: Am J Reprod Immunol. 2010 Mar 4;64(1):12–19. doi: 10.1111/j.1600-0897.2010.00815.x

Fig. 4.

Fig. 4

Cytokine secretion of endothelial cells and apoptotic trophoblast coculture in vitro. H8 cells were cocultured with human endometrial endothelial cells (HEECs) monolayered in EBM-2 supplemented with 10% fetal bovine serum and 800 ng/mL puromycin. After 6, 12, 24, and 48 hr of coculture, cell-free supernatant was collected, and cytokine concentration was measured using LUMINEX (Austin, TX, USA). The cytokine level of HEECs and H8 cells coculture group is minus with the cytokine level of H8 cells single culture control group. Data are presented as mean ± S.D.; *P < 0.05. (a) Interleukin-6; (b) monocyte chemoattractant protein-1.