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. Author manuscript; available in PMC: 2011 Jan 24.
Published in final edited form as: J Immunol. 2008 May 1;180(9):6035–6043. doi: 10.4049/jimmunol.180.9.6035

FIGURE 5.

FIGURE 5

Expression of TLR6 inhibits PDG-induced trophoblast cell death. A, TLR6 mRNA expression was evaluated by RT-PCR in the wild-type trophoblast cells and in trophoblast cells stably transfected to express TLR6. Top panel, TLR6 expression (500 bp) and bottom panel, β-actin (620 bp). Cells were routinely monitored for TLR6 expression and only the stably transfected cells (TLR6+) were positive for this receptor. B, Wild-type or the TLR6+ trophoblast cells were incubated with PDG at 0, 40, or 80 μg/ml for 72 h. Cell viability was then determined using the CellTiter 96 assay. Bar graph, Percentage of cell viability relative to the untreated control (0). Treatment with PDG significantly reduced the wild-type trophoblast cell viability in a dose-dependent manner (*, p < 0.05; **, p < 0.001). Expression of TLR6 significantly inhibited the effect of PDG on trophoblast cell viability when compared with the responses of the wild-type cells (**, p < 0.001).