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Expression of TLR6 results in PDG-induced NF-κB activation in trophoblast cells. Wild-type trophoblast cells or the TLR6+ trophoblast cells were transiently transfected with pBII-LUC. Cells were then treated with or without PDG (40 μg/ml) for 0 and 30 min, after which cell lysates were collected and assayed for NF-κB activity using the luciferase assay. Bar graph, PDG-induced NF-κB activity in RLU after normalization against the untreated controls.
