Table 3.
List of identified proteins isolated using lectin affinity chromatography
| Gene locusa | Lectin | MW [kDa] | pI | ΣGlycoSiteEb | GlycoSitePc | PSORTbd | LipoPe |
|---|---|---|---|---|---|---|---|
| FTH_1651 | ConA, DSA, PNA, SBA, SNA | 57.40 | 4.72 | 3 | - | cyt | - |
| FTH_1722 | ConA, DSA, PNA, SBA, SNA | 65.86 | 6.14 | 1 | DVN581MS | ? | - |
| FTH_0310 | ConA, DSA, PNA, SBA, SNA | 100.27 | 5.65 | 1 | - | cyt | - |
| FTH_0295 | ConA, DSA, PNA, SBA, SNA | 35.44 | 8.07 | 1 | - | ? | - |
| FTH_1719 | ConA, DSA, PNA, SBA, SNA | 52.75 | 4.89 | 2 | - | cyt | - |
| FTH_0384 | ConA, DSA, PNA, SBA, SNA | 13.48 | 9.22 | 2 | - | ? | SPI |
| FTH_1732 | ConA, DSA, PNA, SBA, SNA | 49.87 | 4.89 | 0 | - | cyt | - |
| FTH_0159 | ConA, DSA, PNA, SBA, SNA | 30.22 | 6.10 | 2 | - | cyt | - |
| FTH_0880 | ConA, DSA, PNA, SBA, SNA | 9.47 | 10.59 | 1 | - | ? | - |
| FTH_0414 | ConA, DSA, PNA, SBA, SNA | 15.77 | 9.67 | 0 | - | ? | SPII |
| FTH_1708 | ConA, DSA, PNA, SBA, SNA | 102.70 | 5.30 | 4 | - | cyt | - |
| FTH_1721 | ConA, DSA, PNA, SBA, SNA | 26.57 | 8.18 | 1 | - | cyt | - |
| FTH_1503 | ConA, DSA, PNA, SBA, SNA | 69.85 | 10.14 | 4 | - | CM | - |
| FTH_0570 | ConA, DSA, PNA, SBA, SNA | 19.79 | 9.74 | 1 | - | ? | SPI |
| FTH_1830 | ConA, DSA, PNA, SBA, SNA | 39.75 | 4.49 | 4 | DFN5DS | cyt | - |
| FTH_0357 | ConA, DSA, PNA, SBA, SNA | 21.99 | 5.43 | 2 | - | cyt | - |
| FTH_0039 | ConA, DSA, PNA, SBA, SNA | 15.30 | 5.16 | 1 | - | ? | SPII |
| FTH_0219 | ConA, DSA, PNA, SBA | 26.42 | 8.94 | 0 | - | ? | - |
| FTH_1113 | ConA, DSA, PNA, SNA | 12.89 | 4.28 | 1 | EKN18MS | cyt | - |
| FTH_0228 | ConA, DSA, PNA, SNA | 17.82 | 10.82 | 0 | - | cyt | - |
| FTH_1617 | ConA, DSA, PNA, SNA | 38.46 | 9.50 | 5 | - | CM | SPI |
| FTH_0334 | ConA, DSA, PNA, SNA | 23.29 | 4.95 | 0 | - | OM | SPII |
| FTH_0312 | ConA, DSA, PNA, SNA | 50.53 | 5.87 | 0 | - | cyt | - |
| FTH_0620 | ConA, DSA, PNA, SNA | 18.46 | 5.83 | 0 | - | cyt | - |
| FTH_1293 | ConA, DSA, PNA, SNA | 41.36 | 5.43 | 4 | DSN309IS | OM | SPI |
| FTH_1760 | ConA, DSA, SBA, SNA | 87.36 | 5.09 | 4 | - | ? | - |
| FTH_0592 | ConA, DSA, SBA, SNA | 65.71 | 8.17 | 4 | DDN158ET EEN351IS |
CM | - |
| FTH_1377 | ConA, DSA, SBA, SNA | 44.26 | 9.77 | 1 | - | ? | - |
| FTH_0836 | ConA, DSA, SBA, SNA | 12.87 | 10.55 | 1 | - | ? | - |
| FTH_1071 | ConA, DSA, SBA, SNA | 39.55 | 4.67 | 3 | - | ? | SPII |
| FTH_1686 | DSA, PNA, SBA, SNA | 18.73 | 9.54 | 0 | - | ? | - |
| FTH_1734 | DSA, PNA, SBA, SNA | 55.54 | 4.68 | 0 | ? | - | |
| FTH_0236 | DSA, PNA, SBA, SNA | 12.20 | 10.80 | 0 | - | ? | - |
| FTH_1837 | DSA, PNA, SBA, SNA | 21.08 | 7.07 | 1 | ESN21LS | CM | - |
| FTH_0098 | DSA, PNA, SBA, SNA | 13.73 | 9.02 | 3 | - | ? | SPI |
| FTH_0311 | DSA, PNA, SBA, SNA | 56.79 | 4.79 | 1 | - | CM | - |
| FTH_0827 | ConA, DSA, SBA | 39.19 | 9.02 | 2 | DEN68IT | cyt | - |
| FTH_0257 | ConA, DSA, SBA | 16.78 | 10.72 | 1 | - | ? | - |
| FTH_1761 | ConA, DSA, SBA | 46.28 | 5.55 | 2 | - | cyt | - |
| FTH_0604 | ConA, DSA, SBA | 32.98 | 6.51 | 4 | - | ? | - |
| FTH_1855 | ConA, DSA, SBA | 33.82 | 5.74 | 2 | - | ? | SPI |
| FTH_0172 | ConA, DSA, SBA | 61.95 | 8.74 | 5 | ETN72FS | CM | SPI |
| FTH_1612 | ConA, DSA, SBA | 50.09 | 10.12 | 3 | - | CM | - |
| FTH_0593 | ConA, DSA, SBA | 23.78 | 10.21 | 0 | - | CM | SPI |
| FTH_1167 | ConA, DSA, SNA | 69.18 | 4.62 | 3 | ERN417TT | PP | - |
| FTH_0253 | ConA, DSA, SNA | 13.38 | 11.54 | 0 | - | cyt | - |
| FTH_1764 | ConA, DSA, SNA | 25.20 | 6.95 | 0 | - | cyt | - |
| FTH_0719 | DSA, PNA, SBA | 95.95 | 7.12 | 6 | DVN302SS ETN791QT |
cyt | - |
| FTH_0232 | DSA, PNA, SBA | 22.55 | 10.29 | 0 | - | ? | - |
| FTH_1691 | DSA, SBA, SNA | 43.39 | 4.87 | 1 | - | cyt | - |
| FTH_1216 | DSA, SBA, SNA | 50.31 | 10.31 | 0 | - | ? | - |
| FTH_1763 | DSA, SBA, SNA | 47.59 | 7.06 | 2 | - | cyt | - |
| FTH_0187 | PNA, SBA, SNA | 76.20 | 8.58 | 1 | - | CM | - |
| FTH_0104 | DSA, PNA | 58.90 | 4.48 | 3 | - | ? | - |
| FTH_0234 | DSA, PNA | 30.40 | 11.54 | 1 | - | ? | - |
| FTH_0585 | DSA, SBA | 84.19 | 8.45 | 3 | - | ? | - |
| FTH_1599 | DSA, SBA | 49.32 | 4.33 | 2 | - | ? | SPI |
| FTH_1733 | DSA, SBA | 33.19 | 8.87 | 2 | - | ? | - |
| FTH_1424 | DSA, SBA | 70.74 | 5.42 | 2 | DTN36GS | CM | - |
| FTH_1696 | DSA, SBA | 57.78 | 8.36 | 1 | - | ? | - |
| FTH_0447 | DSA, SBA | 32.13 | 9.72 | 0 | - | ? | - |
| FTH_0886 | DSA, SBA | 40.42 | 8.97 | 3 | DDN41ES | ? | - |
| FTH_1736 | DSA, SBA | 17.38 | 7.54 | 1 | DIN4IT | cyt | - |
| FTH_0826 | DSA, SBA | 53.44 | 9.24 | 4 | - | cyt | - |
| FTH_0139 | DSA, SBA | 49.45 | 4.72 | 1 | - | cyt | - |
| FTH_1609 | DSA, SBA | 66.68 | 8.69 | 6 | DGN371VT | CM | SPI |
| FTH_1536 | DSA, SBA | 25.35 | 6.96 | 4 | - | ? | - |
| FTH_0184 | DSA, SBA | 64.28 | 7.63 | 3 | - | CM | - |
| FTH_1735 | DSA, SBA | 19.20 | 6.11 | 1 | - | cyt | - |
| FTH_1558 | DSA, SBA | 36.08 | 8.69 | 1 | - | CM | - |
| FTH_1379 | DSA, SBA | 44.90 | 7.41 | 2 | - | ? | - |
| FTH_0887 | DSA, SBA | 34.59 | 9.97 | 1 | - | ? | - |
| FTH_1662 | DSA, SBA | 23.83 | 9.93 | 3 | - | ? | - |
| FTH_0543 | DSA, SBA | 22.44 | 5.34 | 0 | - | ? | - |
| FTH_0589 | DSA, SBA | 33.26 | 9.53 | 0 | - | ? | - |
| FTH_0111 | DSA, SBA | 24.59 | 4.86 | 2 | - | ? | - |
| FTH_0186 | DSA, SBA | 34.46 | 7.02 | 2 | - | CM | - |
| FTH_0628 | DSA, SBA | 35.58 | 7.16 | 0 | - | ? | - |
| FTH_0838 | DSA, SBA | 34.48 | 6.28 | 2 | - | CM | - |
| FTH_0837 | DSA, SBA | 69.67 | 9.52 | 2 | - | CM | - |
| FTH_0110 | DSA, SBA | 44.64 | 4.55 | 5 | - | ? | - |
| FTH_1117 | DSA, SBA | 37.84 | 9.62 | 2 | - | ? | - |
| FTH_0423 | SBA, SNA | 33.44 | 7.53 | 0 | - | EC | - |
| FTH_0151 | DSA | 25.39 | 6.06 | 2 | EDN44LT | cyt | - |
| FTH_1112 | DSA | 44.02 | 5.72 | 1 | - | cyt | - |
| FTH_1078 | SBA | 16.87 | 7.40 | 0 | - | ? | - |
| FTH_0327 | SBA | 30.12 | 7.48 | 1 | - | CM | - |
| FTH_0853 | SBA | 50.85 | 9.46 | 0 | - | CM | - |
| FTH_1329 | SBA | 26.43 | 8.75 | 1 | - | cyt | - |
| FTH_1726 | SBA | 47.16 | 9.78 | 1 | - | CM | - |
| FTH_0539 | SBA | 35.44 | 6.05 | 4 | - | cyt | - |
| FTH_0828 | SBA | 21.78 | 10.43 | 1 | - | CM | - |
| FTH_1462 | SBA | 48.02 | 8.51 | 2 | - | CM | - |
| FTH_0541 | SBA | 28.13 | 8.37 | 0 | - | cyt | - |
| FTH_0251 | SBA | 48.46 | 10.01 | 0 | - | CM | - |
| FTH_1168 | SBA | 43.99 | 8.35 | 4 | - | cyt | - |
| FTH_1245 | SBA | 34.91 | 6.97 | 2 | - | cyt | - |
| FTH_0799 | SBA | 150.39 | 7.97 | 4 | ESN1149IS | cyt | - |
| FTH_1373 | SBA | 40.83 | 10.24 | 2 | - | CM | - |
| FTH_0174 | SBA | 36.14 | 9.83 | 0 | - | CM | - |
| FTH_1478 | SBA | 45.43 | 8.25 | 2 | - | CM | - |
| FTH_1047 | SBA | 67.39 | 7.25 | 1 | - | cyt | - |
| FTH_0117 | SBA | 127.47 | 9.63 | 8 | - | OM | - |
| FTH_0397 | SBA | 28.00 | 8.33 | 2 | - | ? | - |
a
the accesion number in the genome sequence of F. tularensis subsp. holarctica OSU18
b
the number of eukaryotic N-glycosylation motifs obtained from NetNGlyc (http://www.cbs.dtu.dk/services/NetNGlyc/)
c
prokaryotic N-glycosylation motif obtained by manual inspection of protein sequence (the index indicates the position of asparagine within the protein sequence)
d
prediction of the protein localization using PSORTb program (http://psort.org/psortb), cyt – cytoplasmic, CM cytoplasmic membrane, OM – outer membrane, PP – periplasm, EC – extracellular space, ? – unknown localization
e
prediction of lipoproteins (SPII cleavage site II) and SPI (cleavage site I) using LipoP algorithm (http://www.cbs.dtu.dk/services/LipoP/)
The accession numbers written in bold represent immunoreacted antigens (from study by Janovska et al30).