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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Cell Death Differ. 2010 Mar 26;17(10):1529–1539. doi: 10.1038/cdd.2010.28

Figure 5. Bid as putative cathepsin B target in SPARC-induced apoptosis.

Figure 5

PNET cells were infected with mock, 100 MOI of Ad-DsRed, and the indicated MOI of Ad-DsRed-SP for 48 h. (A) Cells were harvested and lysed, and fractions of cytoplasmic, mitochondrial and total cell lysates were collected separately. These fractions were used for Western blot analysis for cytochrome c and t-Bid. α-Tubulin served as a loading control for cytosolic fraction and Cox-IV served as loading control from mitochondrial fractions. (B) Cells were harvested after the treatment with cathepsin B inhibitor, and the cytochrome c in the cytosolic fraction was assessed by Western blot analysis using anti-cytochrome c antibody. (C) Densitometric analysis for cytochrome c. (D) PNET cells were infected with Ad-DsRed-SP at the indicated MOI for 24 h, the cells were then transfected with Bid siRNA and cultured for another 24 h. Cells were harvested and Western blot analysis was performed for cytochrome c in the cytosolic fraction. Caspase-3, PARP, LC3 and t-Bid were determined in total cell lysates. GAPDH served as a loading control. Results are representative of three independent experiments. (Columns, mean of three experiments; bars, SD)