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Skp2 siRNA induced p27kip1 accumulationin in PFC in vivo by immunofluorescence. Immunofluorescence staining indicated the expression of p27kip1 dramatically increased in PFC and NFC transfection with Skp2 siRNA (C, F) when compared with PFC transfection with pSuppressor vehicle (A, D) or without transfection (B, E). The scale bar is equal to 40 μm.
