Table 1.

Formerly and newly identified^‡ exported proteins that may be associated with the virulence phenotype of Corynebacterium pseudotuberculosis strains

Protein Descriptiona	GenBank Accession	Identified in the exoproteome of the strainb:		Orhologs found in other Corynebacteriac:		References
		1002	C231	Pathogenic	Non-pathogenic
Phospholipase D (PLD)	ADL09524.1	No	Yes	Yes	No	[54]
Iron siderophore binding protein (FagD)	ADL09528.1	No	Yes	Yes	Yes	[6]
Serine proteinase precursor (CP40)	ADL11339.1	No	Yes	No	No	[57]
Putative iron transport system binding (secreted) protein	ADL10460.1	No	Yes	Yes	No	[12]
Glycerophosphoryl diester phosphodiesterase	ADL11410.1	No	Yes	Yes	No	This work. [72]
Putative surface-anchored membrane protein	ADL20074.1	Yes	Yes	Yes	No	This work.
Putative hydrolase (lysozyme-like)	ADL20788.1	Yes	Yes	Yes	No	This work.
Putative secreted protein	ADL21714.1	Yes	Yes	Yes	No	This work.
Putative sugar-binding secreted protein	ADL09872.1	No	Yes	Yes	No	This work.

^‡ The inclusion criteria followed three main requisites: (i) experimental detection of the proteins in the exoproteomes of the pathogenic C. diphtheriae and C. jeikeium; (ii) non-detection of the proteins in the exoproteomes of the non-pathogenic C. glutamicum and C. efficiens; and (iii) in silico detection of ortholog proteins in pathogenic, but not in non-pathogenic, corynebacteria through search of similarity against public protein repositories.

^a This protein list is not meant to be all-inclusive. Rather, it wants to give an overview of the exported proteins identified in this study for which it was possible to speculate on a probable involvement in C. pseudotuberculosis virulence after comparative proteomic analyses.

^b Proteins identified in this study by TPP/LC-MS^E

^c Searches of similarity against publicly available protein databases using Blast-p.