Figure 3.

Binding efficiency of pig anti-CSFV sera with different rE2-AD proteins. (A) Binding of the rE2-AD proteins from the C-strain and eight subgroup 2.1 strains to pig antisera collected at 78 days post immunization with the C-strain or 25 days post infection with strain QZ-07, respectively. For each of the rE2-AD proteins, the binding efficiency was determined by normalizing to anti-His-tag binding first, and then to C-strain protein or strain QZ-07 rE2-AD protein binding for anti-C-strain or anti-QZ-07 sera, respectively. Thus homologous binding efficiency was set at 100%. Error bars represent standard deviation from three separate experiments. (B) Western blots of rE2-AD proteins using pig anti-C-strain serum, pig anti-QZ-07 serum and mouse monoclonal anti-His-tag antibody.