Table 1.
Primers used in PCR amplification of various recombinant E2 proteins
| Primer designationa | Nucleotide sequenceb | Target region of E2 proteinc | CFSV strain amplified | Location in the C-strain genomed |
|---|---|---|---|---|
| C-E2-BC-f | 5-AAAGGATCCATGCGCTTAGCCTGCAAGGAAGATTAC | BC unit | 2442-2465 | |
| C-E2-BC-r | 5-AAACTCGAGTCAGAAAGCACTACCG | BC unit | 2804-2816 | |
| C-E2-AD-f | 5-AAGGATCCATGCGGCTAGCCTGCAAG | BC + AD units | Vaccine C-strain | 2442-2456 |
| C-E2-AD-r | 5-TAGCTCGAGTCAATCTTCATTTTCCAC | BC + AD units | 2955-2969 | |
| C-E2-f | 5-TTTGGATCCGCCACCATGGTATTAAGGGGACAGATCG | Full-size E2 | 2379-2397 | |
| C-E2-r | 5-ATTCTCGAGTCAACCAGCGGCGAGTTGTTCTG | Full-size E2 | 3541-3560 | |
| QZ-E2-AD-f | 5-AAAGGATCCCGCCTGTCCTGTAAGG | BC + AD units | Subgroup 2.1 Strains | 2442-2457 |
| QZ-E2-AD-r | 5-TAGCTCGAGGTCTTCTTTTTCTAC | BC + AD units | 2955-2969 |
af, forward; r, reverse.
bUnderline represents the restriction enzyme digestion sites used for cloning.
cSee Figure 1 for the various regions of E2 protein.
dLocations are derived from the genome of classical swine fever virus C-strain (GenBank accession no. HM175885).