Figure 1.

Developmental cannabinoid exposure alters CB₁ immunostaining within various zebra finch brain regions. Initial daily treatments over 25 days are indicated by first designations (VEH-, WIN-). Later, single acute treatments in adulthood are indicated second (-VEH, -WIN, see Table 1). Treatments were delivered during sensorimotor song learning (from 50-75 days) and measured in adulthood (> 100 days). Basal levels of staining are decreased following repeated WIN exposure during development in all regions but DLM (compare VEH-VEH to WIN-VEH). Acute responsiveness is increased in all regions (compare VEH-WIN to WIN-WIN). Asterisks indicate differences from VEH-VEH treatment groups (p < 0.05, one-way ANOVA followed by SNK post-tests). Daggers indicate differences from VEH-WIN groups, double-daggers indicate differences from WIN-VEH.