Table 1.
PCR amplification success of DNA extracted from different types/brand of archived RDTs
| Category of RDTs sampled | No. of RDTs | Type/brand of RDT | Mean storage duration in days(range) | PCR results | |
|---|---|---|---|---|---|
| Positive (%) | Negative (%) | ||||
| True positive: RDT +ve/BS +ve | 26 | Paracheck Pf® | 28(7-58) | 26(100) | 0(0) |
| True positive: RDT +ve/BS +ve | 45 | ParaHIT ®f | 47(6-95) | 44(97.8) | 1(2.2) |
| False negative: RDT -ve/BS +ve | 63 | ParaHIT ®f | 60(61-66) | 52(82.5) | 11(17.4)* |
| False positive: RDT +ve/BS -ve | 24 | ParaHIT ®f | 60(55-66) | 4(16.7) | 20(83.3) |
| True negative: RDT -ve/BS -ve | 7 | ParaHIT ®f | 65(63-69) | 0(0) | 7(100) |
RDT = Rapid Diagnostic Test, BS = Blood smear, +ve = positive and -ve = negative
*These samples had low parasite density detected by microscopy (less than 100 asexual parasites/μ; ranged from 16-80 asexual parasites/μl).