Figure 1. Comparison of MDCK cell growth in different culture medium.

Three commercially available serum-free media (Plus-MDCK, VP-SFM, and ExCell) and one SC medium (DMEM+5% FBS) were used to evaluate the suitability for MDCK cells directly grown in 75 cm² T-flasks. The thawed MDCK cells were directly cultured in four different media over 3 passages. The cell numbers were determined based on the harvest when the cell grown in SC had reached 90% confluency in day 3. The cell count in each medium was performed in triplicate. The increase of cell growth (number of fold increase) is calculated based on the final total cell number divided by the initial cell number.