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. 2011 Jan 24;6(1):e16439. doi: 10.1371/journal.pone.0016439

Figure 5. Deletion of Movam7 resulted in altered distribution of chitins.

Figure 5

(A) The ΔMovam7 mutants (#1 and #10), complemented mutant (ΔMovam7R), and wild type strains were incubated on CM plates supplemented with various stress inducers at 28°C for 6 days. Growth of the ΔMovam7 mutant in media supplemented with SDS (0.01%; vs wild type P<0.01), lysing enzymes (2 mg/ml), and Congo Red (20 µg/ml). (B) Deletion of Movam7 altered the distribution of chitin within the cell. In the wild-type strain Guy11, CFW fluorescence was mainly distributed at the hyphal and septal apices, whereas in the ΔMovam7 mutant, fluorescence was not restricted to growing apices and found also on the lateral walls along the hyphal axe. This abnormal distribution of cell wall components was restored by the introduction of the wild-type MoVAM7 gene. (C) Reduced expression was found in six out of seven genes that encode chitin synthases in the ΔMovam7 mutants of M. oryzae. RNA was extracted from mycelia that were grown for 3 days in liquid CM. Error bars represent the standard deviation and asterisks represent significant different among stains tested. All of the reductions are significant (P = 0.01 or P = 0.05) according to Duncan's multiple range test.