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. 2011 Jan 24;6(1):e16427. doi: 10.1371/journal.pone.0016427

Figure 2. Independent and non-overlapping areas of LZAP are sufficient for association with p38.

Figure 2

U2OS cells were transfected with plasmids directing expression of Myc3-tagged truncation mutants of LZAP and full-length Flag-tagged p38. P38 was immunoprecipitated using anti-Flag and LZAP truncation mutants were immunoprecipitated using anti-Myc antibodies before immunoblotting with anti-Myc to detect LZAP truncations or anti-p38. Expression of LZAP truncation mutants was confirmed by immunoblotting and binding activity of LZAP truncation mutants is schematically summarized.