Table 1. Sequences, Contour Lengths, and MICs of HP(2–20) and Its Four Analogures.
AMP | Sequence* | MIC (µM) | Contour length‡ (nm) | |||||
C.albicans | S.aureus | E.coli | ||||||
Ref.31 | Ref.8† | Ref.31 | Ref.8† | Ref.31 | Ref.8† | |||
HP(2–20) | AKKVFKRLEKLFSKIQNDK | >25 | 25 | 12.5 | 6.25 | 3.13 | 6.25 | 3.20±0.05 |
HPA1 | AKKVFKRLEKLFSKIQNWK | 25 | 25 | 3.13 | 3.12 | 0.78 | 3.12 | 2.93±0.03 |
HPA2 | AKKVFKRLEKLFSKIWNDK | 25 | 25 | 6.25 | 3.12 | 3.13 | 3.12 | 2.96±0.07 |
HPA3 | AKKVFKRLEKLFSKIWNWK | 12.5 | 6.25 | 1.56 | 0.78 | 1.56 | 1.56 | 3.16±0.06 |
HPA5 | AKKVSKRLEKLFSKIQNDK | >25 | >100 | >12.5 | 50 | 6.25 | 6.25 | 2.91±0.05 |
*The italic letters in the sequence column indicate the substituted amino acid residues;
The MIC of bacterial growth were measured in low-salt buffer;
The original contour lengths were presented as mean±SE of five different lengths measured from five random conformations in equilibrium for each peptide.