Fig 1.
Expression of ferret CMO2 in Sf9 insect cells. Ferret CMO2 was expressed in Sf9 insect cells using a baculovirus system as described under “Experimental Procedures.” The cell lysates from uninfected (lane 1) and ferret CMO2 baculovirus-infected (lane 2) insect cells were boiled in reducing sample buffer and subjected to 10% SDS-PAGE. The protein expression was detected by Coomassie Blue stain (A) and by detection with a CMO2-specific polyclonal antibody after transfer to a polyvinylidene difluoride membrane (B).
