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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Exp Hematol. 2010 Nov 22;39(2):151–166.e1. doi: 10.1016/j.exphem.2010.11.007

Figure 2. Reduced expression of CXCL12 in the marrows of annexin 2-deficient animals.

Figure 2

Figure 2

Figure 2

Figure 2

(A) Immunohistochemistry for annexin 2 and CXCL12 were performed on the long bones of anxa2 +/+ and anxa2−/− animals using monoclonal antibodies (mAbs) or isotype-matched immunoglobulin G (IgG) controls. Original magnification at 20x. Bar = 100 microns. (B) Immunohistochemistry for annexin 2 and CXCL12 were performed on the primary murine osteoblasts derived from anxa2 +/+ and anxa2−/− animals using the monoclonal antibodies (mAbs) or isotype-matched immunoglobulin G (IgG) controls. Original magnification at 40x. Bar = 100 microns. (C) The CXCL12 mRNA expression was examined by real-time RT-PCR for quantification of CXCL12 expression on the primary murine osteoblasts in Figure 2B. (D) CXCR4 and CXCR7 mRNA expression by HSCs isolated from anxa2 +/+ or anxa2−/− animals were examined by real-time RT-PCR. Data are presented as the mean ± standard error of the mean. *P< 0.001 significant differences from anxa2 +/+ control groups.