Table 1. Mobilization of hematopoietic progenitor cells in response to G-CSF.

To determine if CXCL12 binding to annexin 2 regulates HSC mobilization, anxa2^+/+ and anxa2^−/− animals were administrated G-CSF by intraperitoneal injection over five days whereupon bone marrow and blood cells were collected from anxa2^+/+ and anxa2^−/− animals (n = 5). Cells (2 × 10⁵ cells) were plated onto 6 well plates in a methylcellulose medium for the hematopoietic colony-forming cell assays. BFU-E, CFU-GM, CFU-GEMM, and the total progenitor colony numbers (CFU-C) were counted at 14 day after plating. The total progenitor colony numbers (CFU-C) were presented in Table 1 and BFU-E, CFU-GM and CFU-GEMM data is presented in Supplemental Table 1. The average of progenitor colonies in bone marrow (BM Vehicle), peripheral blood (PB Vehicle), bone marrow with G-CSF treatment (BM G-CSF), and peripheral blood with G-CSF treatment (PB G-CSF) were enumerated. Delta PB (Δ PB) was calculated by the average percent of progenitor colonies in PB Vehicle normalized to the numbers of progenitor colonies in PB G-CSF. Delta PB/BM (Δ PB/BM) was calculated by the average percent values in Delta PB (Δ PB) normalized to the numbers of progenitor colonies in BM vehicle. Data are presented as the mean ± standard error of the mean.

	BM Vehicle	BM G-CSF--	PB Vehicle--	PB G-CSF--	PB	Δ PB/BM
Anxa2 +/+	146 ± 10.0*	103.6 ± 3.2*	8.1 ± 2.2	17.5 ± 1.0 *	55.0 ± 1.0	37.7 ± 0.7*
Anxa2 −/−	99.6 ± 9.2	76.6 ± 7.7	8.3 ± 1.9	31.8 ± 0.9	55.1 ± 1.1	55.2 ± 1.1

^*P< 0.001 significant differences from anxa2^+/+ wild-type control groups.