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HOXB13 is an androgen receptor repressor in presence of androgen. (A) LNCaP cells were transiently transfected with 100 ng of pPSA-luc, 2 ng of renilla, and 100 ng of pFLAG-HOXB13 with or without 10 nM R1881, synthetic androgen. pFLAG-CMV was used as a counterpart of pFLAG-HOXB13. (B) similar to (A) except that pARE4-luc (100 ng) was used. Luciferase assays were performed 48 hours post-transfection. Values indicate as relative luciferase unit (RLU). Each bar represents the mean±S.D.
